Laser scanning microscopy is used in biological research to obtain high-resolution, high-contrast imagery of a sample. Laser microscopes can scan samples point by point, resulting in optical sectioning that can be used to construct precise 3D imagery
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.
